Antibody-mediated pure crimson cell aplasia (PRCA) on switching from darbepoetin alfa to epoetin beta: what are the implications?
We report the event of antibody-mediated pure crimson cell aplasia (PRCA) in a 63-year-old man with end-stage renal illness following a swap from darbepoetin alfa to epoetin beta. Haemoglobin ranges started to lower 6 months after the swap.
Rising the epoetin beta dose produced no response and common blood transfusions have been required; PRCA was confirmed and epoetin beta was discontinued.
The affected person responded positively to immunosuppression; after 2 months on prednisone and cyclophosphamide, hemoglobin ranges stabilized and no additional transfusions have been required.
This case highlights the issue in establishing a cause-effect relationship the place multiple erythropoiesis-stimulating agent is concerned.
Antibodies to lively zone protein ERC1 in Lambert-Eaton myasthenic syndrome.
Lambert-Eaton myasthenic syndrome (LEMS) is characterised by fluctuating muscle weak spot and autonomic dysfunction. In 90% of the LEMS sufferers the illness is related to auto-antibodies in opposition to the voltage-gated calcium channels (VGCC).
A number of auto-immune responses in opposition to different antigenic targets have been described to (co)-occur in LEMS sufferers. To determine new LEMS related small cell lung most cancers (SCLC) markers immunoprecipitation with a SCLC cell line was carried out.
We found sturdy immunoreactivity in opposition to the 120 kDa giant ERC1 protein in a single tumor-negative VGCC-positive LEMS affected person. A recombinant ELISA assay and a mobile assay expressing GFP-tagged full size ERC1 have been used to substantiate the presence of auto-antibodies in opposition to ERC1 on this affected person.
Extra testing of 58 LEMS sufferers together with 9 VGCC auto-antibody detrimental LEMS sufferers, 48 myasthenia gravis sufferers, 84 management sufferers with different illnesses and 12 wholesome controls revealed no different instances. ERC1 is subsequently a brand new, however uncommon, antigen in LEMS.
tgf-b1
PRC1 Antibody | WB, IHC
Description: A polyclonal antibody in opposition to PRC1. Acknowledges PRC1 from Human, Mouse, Rat. This antibody is Unconjugated. Examined within the following software: ELISA, WB, IHC
A polyclonal antibody for detection of PRC1 from Human. This PRC1 antibody is for WB, ELISA. It’s affinity-purified from rabbit serum by affinity-chromatography utilizing the particular immunogenand is unconjugated.
The antibody is produced in rabbit by utilizing as an immunogen synthesized peptide derived from half area of human PRC1 protein at amino acid sequence of 460-520
PRC1 Polyclonal Antibody
BACKGROUND
The immunological strategies for detecting antibodies to erythropoiesis-stimulating brokers (ESAs) differ in assay sensitivity. Nevertheless, this parameter, routinely decided in medical assays utilizing a high-affinity non-human polyclonal antibody, offers a one-dimensional evaluation of antibody detection.
We evaluate three broadly used immunological strategies and consider the power of every to detect mature human antibodies and human antibodies attribute of an early immune response.
METHODS
The detection of anti-ESA antibodies was in contrast between a radioimmunoprecipitation (RIP) assay, an electrochemiluminescence (ECL) bridging enzyme-linked immunosorbent assay and a floor plasmon resonance (SPR)-based immunoassay. All three strategies have been validated for sensitivity, specificity and precision.
Specimens from medical research or put up market testing have been categorized as pure crimson cell aplasia (PRCA) or non-PRCA after which analyzed in every technique.
RESULTS
Among the many antibody-mediated PRCA samples, which include excessive affinity neutralizing antibodies, there was sturdy correlation between all strategies.
The outcomes from non-PRCA pattern analysis present excessive correlation between RIP and ECL strategies; nonetheless, variations between the SPR immunoassay and the ECL and RIP have been demonstrated.
The samples that scored constructive within the SPR immunoassay and detrimental by RIP and ECL have been characterised to be of low antibody focus, contained a excessive share of quickly dissociating antibodies, or have been antibodies of the IgM isotype.
CONCLUSIONS
All three immunological strategies are acceptable for detection of antibodies related to antibody-mediated PRCA.
Nevertheless, the SPR immunoassay platform detected an early, low affinity IgG and IgM antibody response in addition to detected and characterised a pathogenic antibody response related to antibody-mediated PRCA.
Description: A polyclonal antibody against PRC1. Recognizes PRC1 from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1:500-2000, ELISA:1:10000-20000
Description: PRC1 Antibody: PRC1 (Protein Regulator of Cytokinesis 1) is a key regulator of cytokinesis and has been identified as a substrate for several cyclin-dependent kinases (CDKs). High levels of PRC1 expression occurs during S and G2/M and drops dramatically after mitosis exit and G1 entry. It is located in the nucleus during interphase and binds to the midzone of mitotic spindles during anaphase and is localized to the cell midbody during cytokinesis. Depletion of PRC1 has been shown to prevent cellular cleavage, but it has no effect on nuclear division, demonstrating the importance of PRC1 in mitosis.
Description: PRC1 Antibody: PRC1 (Protein Regulator of Cytokinesis 1) is a key regulator of cytokinesis and has been identified as a substrate for several cyclin-dependent kinases (CDKs). High levels of PRC1 expression occurs during S and G2/M and drops dramatically after mitosis exit and G1 entry. It is located in the nucleus during interphase and binds to the midzone of mitotic spindles during anaphase and is localized to the cell midbody during cytokinesis. Depletion of PRC1 has been shown to prevent cellular cleavage, but it has no effect on nuclear division, demonstrating the importance of PRC1 in mitosis.
Description: A polyclonal antibody for detection of PRC1 from Human. This PRC1 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PRC1 protein at amino acid sequence of 460-520
Description: A polyclonal antibody for detection of PRC1 from Human. This PRC1 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PRC1 protein at amino acid sequence of 460-520
Description: A polyclonal antibody for detection of PRC1 from Human. This PRC1 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PRC1 protein at amino acid sequence of 460-520
Description: This gene encodes a protein that is involved in cytokinesis. The protein is present at high levels during the S and G2/M phases of mitosis but its levels drop dramatically when the cell exits mitosis and enters the G1 phase. It is located in the nucleus during interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly. Alternative splicing results in multiple transcript variants.
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PRC1 . This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PRC1 (Internal). This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody against Phospho-PRC1 (Thr481). Recognizes Phospho-PRC1 (Thr481) from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1:500-2000, ELISA:1:10000-20000
Description: A polyclonal antibody for detection of PRC1 Phospho-Thr481) from Human. This PRC1 Phospho-Thr481) antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PRC1 (Phospho-Thr481) Polyclonal Antibody protein at amino acid sequence of 481
Description: A polyclonal antibody for detection of PRC1 Phospho-Thr481) from Human. This PRC1 Phospho-Thr481) antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PRC1 (Phospho-Thr481) Polyclonal Antibody protein at amino acid sequence of 481
Description: A polyclonal antibody for detection of PRC1 Phospho-Thr481) from Human. This PRC1 Phospho-Thr481) antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PRC1 (Phospho-Thr481) Polyclonal Antibody protein at amino acid sequence of 481
Description: A Rabbit Polyclonal antibody against PRC1(Phospho-Thr481)Rabbit from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA
Description: A Rabbit Polyclonal antibody against PRC1(Phospho-Thr481)Rabbit from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA
Description: This gene encodes a protein that’s concerned in cytokinesis. The protein is current at excessive ranges through the S and G2/M phases of mitosis however its ranges drop dramatically when the cell exits mitosis and enters the G1 part.
It’s situated within the nucleus throughout interphase, turns into related to mitotic spindles in a extremely dynamic method throughout mitosis, and localizes to the cell mid-body throughout cytokinesis. This protein has been proven to be a substrate of a number of cyclin-dependent kinases (CDKs).
It’s obligatory for polarizing parallel microtubules and concentrating the components accountable for contractile ring meeting. Various splicing leads to a number of transcript variants.
PRC1 (Phospho- Thr481) Antibody
PR81 is a monoclonal antibody that binds with excessive affinity to MUC1, which is over expressed on breast and different tumors. The target of this research was to guage the software of this antibody in opposition to MUC1 as a radioimmunotherapeutical agent. Monoclonal antibody (PR81) in opposition to MUC1 was ready, characterised, purified, and labeled with 131I.
The immunoreactivity of radiolabeled mAb PR81with MUC1 (the native protein), BSA-P20 (a 20 amino acid corresponding the tandem repeat of MUC1) and MCF7 cell line have been carried out by RIA. In vitro stability of radiolabeled mAb in human serum was decided by skinny layer chromatography (TLC).
Cell toxicity and in vitro internalization research have been carried out with the MCF7 cell line, and the tissue biodistribution of the radioiodinated PR81 was evaluated in regular BALB/c mice at 4, 24 and 48 hrs. The tumor imaging was carried out in BALB/c mice with breast xenograft tumors at 24 and 72 hr after the complicated injection.
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PRC1 (Internal). This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody against PRC1. Recognizes PRC1 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:50-1:200
Description: A polyclonal antibody against PRC1. Recognizes PRC1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC
Description: A polyclonal antibody against PRC1. Recognizes PRC1 from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1:500-2000, ELISA:1:10000-20000
Description: PRC1 Antibody: PRC1 (Protein Regulator of Cytokinesis 1) is a key regulator of cytokinesis and has been identified as a substrate for several cyclin-dependent kinases (CDKs). High levels of PRC1 expression occurs during S and G2/M and drops dramatically after mitosis exit and G1 entry. It is located in the nucleus during interphase and binds to the midzone of mitotic spindles during anaphase and is localized to the cell midbody during cytokinesis. Depletion of PRC1 has been shown to prevent cellular cleavage, but it has no effect on nuclear division, demonstrating the importance of PRC1 in mitosis.
Description: PRC1 Antibody: PRC1 (Protein Regulator of Cytokinesis 1) is a key regulator of cytokinesis and has been identified as a substrate for several cyclin-dependent kinases (CDKs). High levels of PRC1 expression occurs during S and G2/M and drops dramatically after mitosis exit and G1 entry. It is located in the nucleus during interphase and binds to the midzone of mitotic spindles during anaphase and is localized to the cell midbody during cytokinesis. Depletion of PRC1 has been shown to prevent cellular cleavage, but it has no effect on nuclear division, demonstrating the importance of PRC1 in mitosis.
Description: A polyclonal antibody for detection of PRC1 Phospho-Thr481) from Human. This PRC1 Phospho-Thr481) antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PRC1 (Phospho-Thr481) Polyclonal Antibody protein at amino acid sequence of 481
Description: A polyclonal antibody for detection of PRC1 Phospho-Thr481) from Human. This PRC1 Phospho-Thr481) antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PRC1 (Phospho-Thr481) Polyclonal Antibody protein at amino acid sequence of 481
Description: A polyclonal antibody for detection of PRC1 Phospho-Thr481) from Human. This PRC1 Phospho-Thr481) antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PRC1 (Phospho-Thr481) Polyclonal Antibody protein at amino acid sequence of 481
Description: A Rabbit Polyclonal antibody against PRC1(Phospho-Thr481)Rabbit from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA
Description: A Rabbit Polyclonal antibody against PRC1(Phospho-Thr481)Rabbit from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA
Description: This gene encodes a protein that is involved in cytokinesis. The protein is present at high levels during the S and G2/M phases of mitosis but its levels drop dramatically when the cell exits mitosis and enters the G1 phase. It is located in the nucleus during interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly. Alternative splicing results in multiple transcript variants.
Description: A polyclonal antibody against Phospho-PRC1 (Thr481). Recognizes Phospho-PRC1 (Thr481) from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1:500-2000, ELISA:1:10000-20000
